...Lipoylation,Angewandte Chemie International Edition - X-MOL

来自 : www.x-mol.com/paper/1326745348 发布时间：2021-03-25

Protein lipoylation is a post‐translational modification of emerging importance in both prokaryotes and eukaryotes. However, labeling and large‐scale profiling of protein lipoylation remain challenging. Here, we report the development of iLCL (iodoacetamide‐assisted lipoate‐cyclooctyne ligation), a chemoselective reaction that enables chemical tagging of protein lipoylation. We demonstrate that the cyclic disulfide of lipoamide but not linear disulfides can selectively react with iodoacetamide to produce sulfenic acid, which can be conjugated with cyclooctyne probes. iLCL enables tagging of lipoylated proteins for gel‐based detection and cellular imaging. Furthermore, we apply iLCL for proteomic profiling of lipoylated proteins in both bacteria and mammalian cells. In addition to all of the eight known lipoylated proteins, we identified seven candidates for novel lipoylated proteins. The iLCL strategy should facilitate uncovering the biological function of protein lipoylation.

中文翻译：

2020年12月21日Protein lipoylation is a post‐translational modification of emerging importance in both prokaryotes and eukaryotes. However, labeling and l...Chemical Tagging of Protein Lipoylation; Qi Tang, Yilan Guo, Liying Meng, Xing Chen; Angew. Chem. Int. Ed.; Angewandte Chemie International Edition; X-MOLProtein lipoylation is a post‐translational modification of emerging importance in both prokaryotes and eukaryotes. However, labeling and large‐scale profiling of protein lipoylation remain challenging. Here, we report the development of iLCL (iodoacetamide‐assisted lipoate‐cyclooctyne ligation), a chemoselective reaction that enables chemical tagging of protein lipoylation. We demonstrate that the cyclic disulfide of lipoamide but not linear disulfides can selectively react with iodoacetamide tChemical Tagging of Protein Lipoylation,Angewandte Chemie International Edition