...Tubulin,Angewandte Chemie International Edition - X-MOL

来自 : www.x-mol.com/paper/514... 发布时间：2021-03-25

The microtubule‐associated protein Tau promotes the polymerization of tubulin and modulates the function of microtubules. As a consequence of the dynamic nature of the Tau–tubulin interaction, the structural basis of this complex has remained largely elusive. By using NMR methods optimized for ligand–receptor interactions in combination with site‐directed mutagenesis we demonstrate that the flanking domain downstream of the four microtubule‐binding repeats of Tau binds competitively to a site on the α‐tubulin surface. The binding process is complex, involves partial coupling of different interacting regions, and is modulated by phosphorylation at Y394 and S396. This study strengthens the hypothesis of an intimate relationship between Tau phosphorylation and tubulin binding and highlights the power of the INPHARMA NMR method to characterize the interaction of peptides derived from intrinsically disordered proteins with their molecular partners.
2018年2月22日The microtubule‐associated protein Tau promotes the polymerization of tubulin and modulates the function of microtubules. As a consequence ...The Binding Mode of a Tau Peptide with Tubulin; Harindranath Kadavath,Yunior Cabrales Fontela,Mariusz Jaremko,Łukasz Jaremko,Kerstin Overkamp,Jacek Biernat,Eckhard Mandelkow,Markus Zweckstetter; Angew. Chem. Int. Ed.; Angewandte Chemie International Edition; X-MOLThe microtubule‐associated protein Tau promotes the polymerization of tubulin and modulates the function of microtubules. As a consequence of the dynamic nature of the Tau–tubulin interaction, the structural basis of this complex has remained largely elusive. By using NMR methods optimized for ligand–receptor interactions in combination with site‐directed mutagenesis we demonstrate that the flanking domain downstream of the four microtubule‐binding repeats of Tau binds competitively to a site onThe Binding Mode of a Tau Peptide with Tubulin,Angewandte Chemie International Edition